Globally, 1.8 billion people use drinking water sources contaminated with faeces, and this is a leading cause of diseases such as diarrhoea, cholera, typhoid, and dysentery. Fecal coliform bacteria indicate the presence of sewage contamination of a waterway and the possible presence of other pathogenic organisms. High fecal coliform counts in water indicates that it contains other possible pathogenic strains which can bring about diseases like Typhoid fever, hepatitis, gastroenteritis, dysentery and ear infections. As per WHO guidelines, no fecal coliform should be present in drinking water. Thus, early and rapid detection of fecal coliform bacteria in drinking water with high sensitivity and accuracy, by using an affordable and robust biosensor will help government bodies to take preventive and precautionary measures to avoid health hazards in a community.